Composite
Part:BBa_K4880016:Design
Designed by: Xinran Xia Group: iGEM23_BASIS-China (2023-09-08)
Ptrc-theo-αPS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1361
Illegal XhoI site found at 1441 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our design, the expression of α-pinene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.
Source
This composite part combines BBa_K3930029 and BBa_K517002.